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Oroboros Instruments oxygraph 2k instrument
Oxygraph 2k Instrument, supplied by Oroboros Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/oxygraph-2k+instrument/2k+oxygraph/pm42270976-504-9-11
Average 86 stars, based on 1 article reviews
oxygraph 2k instrument - by Bioz Stars, 2026-09
86/100 stars

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Transfection:

Article Title: Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners.
Article Snippet: For routine respiration, cells were trypsinized, washed with PBS, resuspended inMir05medium (0.5mMEGTA, 3mM MgCl2, 60 mM K-lactobionate, 20mM taurine, 10mM KH2PO4, Nature Communications | (2024) 15:473 10 110mM sucrose, 1 g/l essentially fatty acid-free bovine serum albumin, 20mM Hepes, pH 7.1 at 30 °C) and transferred to the chamber of Oxygraph-2k instrument (Oroboros).

Article Title: Mitotherapy prevents peripheral neuropathy induced by oxaliplatin in mice.
Article Snippet: Oxaliplatin (OXA) is an antineoplastic agent used for the treatment of cisplatin-resistant tumours, presenting lower incidence of nephrotoxicity and myelotoxicity than other platinum-based drugs.. However, OXA treatment is highly associated with painful peripheral neuropathy, a well-known and relevant side effect caused by mitochondrial dysfunction.. The transfer of functional exogenous mitochondria (mitotherapy) is a promising therapeutic strategy for mitochondrial diseases.

Article Title: Altered acylcarnitine metabolism and inflexible mitochondrial fuel utilization characterize the loss of neonatal myocardial regeneration capacity.
Article Snippet: The rate of oxidative phosphorylation (OXPHOS) was measured using an Oxygraph-2k instrument (OROBOROS Instruments Corp., Innsbruck, Austria), as we have previously described2.

Article Title: Childhood adverse life events and skeletal muscle mitochondrial function
Article Snippet: Briefly, an Oxygraph-2k instrument (Oroboros Inc., Innsbruck, Austria) was used to measure the respiration of the PMF bundles.

Article Title: Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners
Article Snippet: For routine respiration, cells were trypsinized, washed with PBS, resuspended in Mir05 medium (0.5 mM EGTA, 3 mM MgCl2, 60 mM K-lactobionate, 20 mM taurine, 10 mM KH2PO4, 110 mM sucrose, 1 g/l essentially fatty acid-free bovine serum albumin, 20 mM Hepes, pH 7.1 at 30 °C) and transferred to the chamber of Oxygraph-2k instrument (Oroboros).

Article Title: Exploration of influenza A virus PA protein-associated cellular proteins discloses its impact on mitochondrial function.
Article Snippet: The mitochondrial respiration in 293T cells transfected with 3×FLAG-tagged PA from the PR8/H1N1 expression plasmid for 48 h was analyzed via an Oxygraph-2k instrument (Oroboros Instrument).

Article Title: 5-Hydroxytryptophan acts as a gap junction inhibitor to limit the spread of chemotherapy-induced cardiomyocyte injury and mitochondrial dysfunction
Article Snippet: Mitochondrial respiration (state 4), i.e., conventional mitochondrial respiration, was initiated by adding 5 mM pyruvate (P) and 2 mM malate (M) to the chamber of the Oxygraph-2k instrument (Oroboros, Austria) [ ]; then 2.5 mM ADP and 3 mM MgCl 2 were added to activate ATP synthase and record mitochondrial oxidative phosphorylation (OXPHOS) levels (state 3) [ ]; followed by the addition of 5 nM oligomycin (Omy), which inhibits ATP synthase to induce a non-phosphorylated leaky state and estimate overall mitochondrial-associated respiration [ ].

Article Title: Effects of Time-Restricted Fasting–Nicotinamide Mononucleotide Combination on Exercise Capacity via Mitochondrial Activation and Gut Microbiota Modulation
Article Snippet: The mitochondrial oxygen consumption rate (OCR) was measured using an Oxygraph-2k instrument (Oroboros, Innsbruck, Austria).

Expressing:

Article Title: Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners.
Article Snippet: For routine respiration, cells were trypsinized, washed with PBS, resuspended inMir05medium (0.5mMEGTA, 3mM MgCl2, 60 mM K-lactobionate, 20mM taurine, 10mM KH2PO4, Nature Communications | (2024) 15:473 10 110mM sucrose, 1 g/l essentially fatty acid-free bovine serum albumin, 20mM Hepes, pH 7.1 at 30 °C) and transferred to the chamber of Oxygraph-2k instrument (Oroboros).

Article Title: Mitotherapy prevents peripheral neuropathy induced by oxaliplatin in mice.
Article Snippet: Oxaliplatin (OXA) is an antineoplastic agent used for the treatment of cisplatin-resistant tumours, presenting lower incidence of nephrotoxicity and myelotoxicity than other platinum-based drugs.. However, OXA treatment is highly associated with painful peripheral neuropathy, a well-known and relevant side effect caused by mitochondrial dysfunction.. The transfer of functional exogenous mitochondria (mitotherapy) is a promising therapeutic strategy for mitochondrial diseases.

Article Title: Altered acylcarnitine metabolism and inflexible mitochondrial fuel utilization characterize the loss of neonatal myocardial regeneration capacity.
Article Snippet: The rate of oxidative phosphorylation (OXPHOS) was measured using an Oxygraph-2k instrument (OROBOROS Instruments Corp., Innsbruck, Austria), as we have previously described2.

Article Title: Childhood adverse life events and skeletal muscle mitochondrial function
Article Snippet: Briefly, an Oxygraph-2k instrument (Oroboros Inc., Innsbruck, Austria) was used to measure the respiration of the PMF bundles.

Article Title: Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners
Article Snippet: For routine respiration, cells were trypsinized, washed with PBS, resuspended in Mir05 medium (0.5 mM EGTA, 3 mM MgCl2, 60 mM K-lactobionate, 20 mM taurine, 10 mM KH2PO4, 110 mM sucrose, 1 g/l essentially fatty acid-free bovine serum albumin, 20 mM Hepes, pH 7.1 at 30 °C) and transferred to the chamber of Oxygraph-2k instrument (Oroboros).

Article Title: Exploration of influenza A virus PA protein-associated cellular proteins discloses its impact on mitochondrial function.
Article Snippet: The mitochondrial respiration in 293T cells transfected with 3×FLAG-tagged PA from the PR8/H1N1 expression plasmid for 48 h was analyzed via an Oxygraph-2k instrument (Oroboros Instrument).

Article Title: 5-Hydroxytryptophan acts as a gap junction inhibitor to limit the spread of chemotherapy-induced cardiomyocyte injury and mitochondrial dysfunction
Article Snippet: Mitochondrial respiration (state 4), i.e., conventional mitochondrial respiration, was initiated by adding 5 mM pyruvate (P) and 2 mM malate (M) to the chamber of the Oxygraph-2k instrument (Oroboros, Austria) [ ]; then 2.5 mM ADP and 3 mM MgCl 2 were added to activate ATP synthase and record mitochondrial oxidative phosphorylation (OXPHOS) levels (state 3) [ ]; followed by the addition of 5 nM oligomycin (Omy), which inhibits ATP synthase to induce a non-phosphorylated leaky state and estimate overall mitochondrial-associated respiration [ ].

Article Title: Effects of Time-Restricted Fasting–Nicotinamide Mononucleotide Combination on Exercise Capacity via Mitochondrial Activation and Gut Microbiota Modulation
Article Snippet: The mitochondrial oxygen consumption rate (OCR) was measured using an Oxygraph-2k instrument (Oroboros, Innsbruck, Austria).

Plasmid Preparation:

Article Title: Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners.
Article Snippet: For routine respiration, cells were trypsinized, washed with PBS, resuspended inMir05medium (0.5mMEGTA, 3mM MgCl2, 60 mM K-lactobionate, 20mM taurine, 10mM KH2PO4, Nature Communications | (2024) 15:473 10 110mM sucrose, 1 g/l essentially fatty acid-free bovine serum albumin, 20mM Hepes, pH 7.1 at 30 °C) and transferred to the chamber of Oxygraph-2k instrument (Oroboros).

Article Title: Mitotherapy prevents peripheral neuropathy induced by oxaliplatin in mice.
Article Snippet: Oxaliplatin (OXA) is an antineoplastic agent used for the treatment of cisplatin-resistant tumours, presenting lower incidence of nephrotoxicity and myelotoxicity than other platinum-based drugs.. However, OXA treatment is highly associated with painful peripheral neuropathy, a well-known and relevant side effect caused by mitochondrial dysfunction.. The transfer of functional exogenous mitochondria (mitotherapy) is a promising therapeutic strategy for mitochondrial diseases.

Article Title: Altered acylcarnitine metabolism and inflexible mitochondrial fuel utilization characterize the loss of neonatal myocardial regeneration capacity.
Article Snippet: The rate of oxidative phosphorylation (OXPHOS) was measured using an Oxygraph-2k instrument (OROBOROS Instruments Corp., Innsbruck, Austria), as we have previously described2.

Article Title: Childhood adverse life events and skeletal muscle mitochondrial function
Article Snippet: Briefly, an Oxygraph-2k instrument (Oroboros Inc., Innsbruck, Austria) was used to measure the respiration of the PMF bundles.

Article Title: Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners
Article Snippet: For routine respiration, cells were trypsinized, washed with PBS, resuspended in Mir05 medium (0.5 mM EGTA, 3 mM MgCl2, 60 mM K-lactobionate, 20 mM taurine, 10 mM KH2PO4, 110 mM sucrose, 1 g/l essentially fatty acid-free bovine serum albumin, 20 mM Hepes, pH 7.1 at 30 °C) and transferred to the chamber of Oxygraph-2k instrument (Oroboros).

Article Title: Exploration of influenza A virus PA protein-associated cellular proteins discloses its impact on mitochondrial function.
Article Snippet: The mitochondrial respiration in 293T cells transfected with 3×FLAG-tagged PA from the PR8/H1N1 expression plasmid for 48 h was analyzed via an Oxygraph-2k instrument (Oroboros Instrument).

Article Title: 5-Hydroxytryptophan acts as a gap junction inhibitor to limit the spread of chemotherapy-induced cardiomyocyte injury and mitochondrial dysfunction
Article Snippet: Mitochondrial respiration (state 4), i.e., conventional mitochondrial respiration, was initiated by adding 5 mM pyruvate (P) and 2 mM malate (M) to the chamber of the Oxygraph-2k instrument (Oroboros, Austria) [ ]; then 2.5 mM ADP and 3 mM MgCl 2 were added to activate ATP synthase and record mitochondrial oxidative phosphorylation (OXPHOS) levels (state 3) [ ]; followed by the addition of 5 nM oligomycin (Omy), which inhibits ATP synthase to induce a non-phosphorylated leaky state and estimate overall mitochondrial-associated respiration [ ].

Article Title: Effects of Time-Restricted Fasting–Nicotinamide Mononucleotide Combination on Exercise Capacity via Mitochondrial Activation and Gut Microbiota Modulation
Article Snippet: The mitochondrial oxygen consumption rate (OCR) was measured using an Oxygraph-2k instrument (Oroboros, Innsbruck, Austria).



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